Overturning paradigms of membrane traffic by yeast live imaging
نویسندگان
چکیده
منابع مشابه
Topic Introduction Live Cell Imaging of Yeast
The development of cloning vectors for green fluorescent protein (GFP) and the simplicity of yeast reverse genetics allow straightforward labeling of yeast proteins in living cells. Budding and fission yeast are therefore attractive organisms in which to study dynamic cellular processes such as growth, cell division, and morphogenesis using live cell fluorescence microscopy. This article focuse...
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Live cell imaging complements the array of biochemical and molecular genetic approaches to provide a comprehensive insight into functional dependencies and molecular interactions in fission yeast. Fluorescent proteins and vital dyes reveal dynamic changes in the spatial distribution of organelles and the proteome and how each alters in response to changes in environmental and genetic compositio...
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Correctly propagating waves from overhanging reflectors is crucial for imaging in complex geology. This type of reflections are difficult or impossible to use in imaging using one-way downward continuation, because they violate an intrinsic assumption of this imaging method, i.e. vertical upward propagation of reflection data. Riemannian wavefield extrapolation is one of the techniques develope...
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We elucidate the interaction between actin and specific membrane components, using real time live cell imaging, by delivering probes that enable access to components, that cannot be accessed genetically. We initially investigated the close interplay between Phosphatidylinositol 4,5-bisphosphate (PIP2) and the F-actin network. We show that, during the early stage of cell adhesion, PIP2 forms dom...
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Eukaryotic cells are composed of an intricate system of internal membranes that are organized into different compartments--including the endoplasmic reticulum (ER), the nuclear envelope, the Golgi complex (GC), lysosomes, endosomes, caveolae, mitochondria, and peroxisomes--that perform specialized tasks within the cell. The localization and dynamics of intracellular compartments are now being s...
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ژورنال
عنوان ژورنال: PLANT MORPHOLOGY
سال: 2018
ISSN: 0918-9726,1884-4154
DOI: 10.5685/plmorphol.30.3